The Alkylquinolone Repertoire of Pseudomonas aeruginosa is Linked to Structural Flexibility of the FabH‐like 2‐Heptyl‐3‐hydroxy‐4(1H)‐quinolone (PQS) Biosynthesis Enzyme PqsBC

Pseudomonas aeruginosa is a bacterial pathogen that causes life‐threatening infections in immunocompromised patients. It produces a large armory of saturated and mono‐unsaturated 2‐alkyl‐4(1H)‐quinolones (AQs) and AQ N‐oxides (AQNOs) that serve as signaling molecules to control the production of virulence factors and that are involved in membrane vesicle formation and iron chelation; furthermore, they also have, for example, antibiotic properties. It has been shown that the β‐ketoacyl‐acyl‐carrier protein synthase III (FabH)‐like heterodimeric enzyme PqsBC catalyzes the last step in the biosynthesis of the most abundant AQ congener, 2‐heptyl‐4(1H)‐quinolone (HHQ), by condensing octanoyl‐coenzyme A (CoA) with 2‐aminobenzoylacetate (2‐ABA), but the basis for the large number of other AQs/AQNOs produced by P. aeruginosa is not known. Here, we demonstrate that PqsBC uses different medium‐chain acyl‐CoAs to produce various saturated AQs/AQNOs and that it also biosynthesizes mono‐unsaturated congeners. Further, we determined the structures of PqsBC in four different crystal forms at 1.5 to 2.7 Å resolution. Together with a previous report, the data reveal that PqsBC adopts open, intermediate, and closed conformations that alter the shape of the acyl‐binding cavity and explain the promiscuity of PqsBC. The different conformations also allow us to propose a model for structural transitions that accompany the catalytic cycle of PqsBC that might have broader implications for other FabH‐enzymes, for which such structural transitions have been postulated but have never been observed.     

Development of planning level transportation safety tools using Geographically Weighted Poisson Regression

A common technique used for the calibration of collision prediction models is the Generalized Linear Modeling (GLM) procedure with the assumption of Negative Binomial or Poisson error distribution. In this technique, fixed coefficients that represent the average relationship between the dependent variable and each explanatory variable are estimated. However, the stationary relationship assumed may hide some important spatial factors of the number of collisions at a particular traffic analysis zone. Consequently, the accuracy of such models for explaining the relationship between the dependent variable and the explanatory variables may be suspected since collision frequency is likely influenced by many spatially defined factors such as land use, demographic characteristics, and traffic volume patterns. The primary objective of this study is to investigate the spatial variations in the relationship between the number of zonal collisions and potential transportation planning predictors, using the Geographically Weighted Poisson Regression modeling technique. The secondary objective is to build on knowledge comparing the accuracy of Geographically Weighted Poisson Regression models to that of Generalized Linear Models. The results show that the Geographically Weighted Poisson Regression models are useful for capturing spatially dependent relationships and generally perform better than the conventional Generalized Linear Models.     

利用聚合物PVT关系在线控制技术的实验研究

为了提高注射成型过程的控制精度,研发了一种利用聚合物PVT（压力-比容-温度）关系在线控制技术,分别在制品质量重复精度、模具温度的变化、制品质量变化、模腔熔体压力和温度的变化情况等方面做了详细的分析和讨论.研究发现,这种在线控制技术利用控制熔体压力和温度关系实现了对熔体比容的控制,从而可以大幅度提高制品质量重复精度.     

Double dissociation in the anatomy of socioemotional disinhibition and executive functioning in dementia.

Objective: To determine whether socioemotional disinhibition and executive dysfunction are related to dissociable patterns of brain atrophy in neurodegenerative disease. Previous studies have indicated that behavioral and cognitive dysfunction in neurodegenerative disease are linked to atrophy in different parts of the frontal lobes, but these prior studies did not establish that these relationships were specific, which would best be demonstrated by a double dissociation. Method: Subjects included 157 patients with neurodegenerative disease. A semiautomated parcellation program (Freesurfer) was used to generate regional cortical volumes from structural MRI scans. Regions of interest (ROIs) included anterior cingulate cortex (ACC), orbitofrontal cortex (OFC), middle frontal gyrus (MFG), and inferior frontal gyrus (IFG). Socioemotional disinhibition was measured using the Neuropsychiatric Inventory. Principal component analysis including 3 tasks of executive function (EF; verbal fluency, Stroop Interference, modified Trails) was used to generate a single-factor score to represent EF. Results: Partial correlations between ROIs, disinhibition, and EF were computed after controlling for total intracranial volume, Mini-Mental State Examination, diagnosis, age, and education. Brain regions significantly correlated with disinhibition (ACC, OFC, IFG, and temporal lobes) and EF (MFG) were entered into separate hierarchical regressions to determine which brain regions predicted disinhibition and EF. OFC was the only brain region to significantly predict disinhibition, and MFG significantly predicted EF performance. A multivariate general linear model demonstrated a significant interaction between ROIs and cognitive–behavioral functions. Conclusions: These results support a specific association between orbitofrontal areas and behavioral management as compared with dorsolateral areas and EF. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Intergenerational cultural dissonance in parent–adolescent relationships among Chinese and European Americans.

Generational cultural gaps (assessed as the mismatch between adolescents' ideals and perceptions of the parent–adolescent relationship) were investigated among Chinese youth with immigrant parents and their European American counterparts who have been in the United States for generations and assumingly do not have intergenerational cultural gaps. The authors of the study examined the associations of such generational gaps with adolescents' behavioral problems and whether youth's appreciation of Chinese parent–adolescent relationships (parental devotion, sacrifice, thoughtfulness, and guan) described by the notion of qin would moderate the relationship between discrepancies and youth's adjustment. A total of 634 high school students (M = 15.97 years; 95 and 154 first- and second-generation Chinese American respectively, and 385 European Americans) completed measures of parental warmth, parent–adolescent open communication, qin, and psychological adjustment. The U.S.-born Chinese American adolescents' ideals exceeded perceptions of parents' warmth and open communication to a greater degree than it did for European American adolescents (ps     

Moral psychology is relationship regulation: Moral motives for unity, hierarchy, equality, and proportionality.

Genuine moral disagreement exists and is widespread. To understand such disagreement, we must examine the basic kinds of social relationships people construct across cultures and the distinct moral obligations and prohibitions these relationships entail. We extend relational models theory (Fiske, 1991) to identify 4 fundamental and distinct moral motives. Unity is the motive to care for and support the integrity of in-groups by avoiding or eliminating threats of contamination and providing aid and protection based on need or empathic compassion. Hierarchy is the motive to respect rank in social groups where superiors are entitled to deference and respect but must also lead, guide, direct, and protect subordinates. Equality is the motive for balanced, in-kind reciprocity, equal treatment, equal say, and equal opportunity. Proportionality is the motive for rewards and punishments to be proportionate to merit, benefits to be calibrated to contributions, and judgments to be based on a utilitarian calculus of costs and benefits. The 4 moral motives are universal, but cultures, ideologies, and individuals differ in where they activate these motives and how they implement them. Unlike existing theories (Haidt, 2007; Hauser, 2006; Turiel, 1983), relationship regulation theory predicts that any action, including violence, unequal treatment, and “impure” acts, may be perceived as morally correct depending on the moral motive employed and how the relevant social relationship is construed. This approach facilitates clearer understanding of moral perspectives we disagree with and provides a template for how to influence moral motives and practices in the world. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Emotional plasticity: Conditions and effects of improving emotional competence in adulthood.

This study aimed to investigate (a) whether it is possible to increase emotional competence (EC) in adulthood; (b) whether this improvement results in better mental, physical, and social adjustment; (c) whether this improvement can be maintained 1 year later; and (d) whether these benefits are accompanied by a reduction in stress-hormone secretion (i.e., cortisol). One hundred and thirty-two participants were randomly assigned to an EC-enhancing intervention (in group format) or to a control group. Participants in the intervention group underwent a specifically designed 15-hr intervention targeting the 5 core emotional competencies, complemented with a 4-week e-mail follow-up. Results reveal that the level of emotional competencies increased significantly in the intervention group in contrast with the control group. This increase resulted in lower cortisol secretion, enhanced subjective and physical well-being, as well as improved quality of social and marital relationships in the intervention group. No significant change occurred in the control group. Peer reports on EC and quality of relationships confirmed these results. These data suggest that emotional competencies can be improved, with effective benefits on personal and interpersonal functioning lasting for at least 1 year. The theoretical implications of these results as well as their practical implications for the construction and the development of effective emotional competencies interventions are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Stigma and intimacy in same-sex relationships: A narrative approach.

Lesbian, gay, and bisexual individuals in romantic relationships experience stigma, prejudice, and discrimination stemming from widespread social devaluation of same-sex relationships. Research on same-sex couples has demonstrated a negative association between experiences of stigma and relationship quality. However, critical questions remain unanswered regarding how experiences of stigma become more or less meaningful within the context of same-sex relationships. This paper presents a study of the stories that a purposive sample of 99 individuals in same-sex relationships wrote about their relational high points, low points, decisions, and goals, as well as their experiences of stigma directly related to their relationships. Narrative analysis of these stories revealed that participants utilized several psychological strategies for making meaning of their experiences of stigma within the context of their relationships. Some participants framed stigma as having a negative impact on their relationships, while others framed stigma as relevant, but external to their lives. Some participants saw stigma as providing an opportunity to (re)define notions of commitment and relational legitimacy. Additionally, many participants framed stigma as bringing them closer to their partners and strengthening the bond within their relationships. The results of this study illuminate the psychological strategies individuals in same-sex couples use to make meaning of, cope with, and overcome societal devaluation thereby furthering understandings of the association between stigma and intimacy within marginalized relationships. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Managing others like you were managed: How prevention focus motivates copying interpersonal norms.

In 5 studies, we investigated the relation between regulatory focus and the tendency to copy a role model's managing behavior after one experiences this behavior as its recipient and later takes on the same managing role. Because enacting role-related behaviors fulfills interpersonal norms that fit prevention concerns, we predicted a stronger tendency to copy among individuals with a stronger prevention focus on duties and obligations (“oughts”) but not among those with a stronger promotion focus on aspirations and advancements (ideals). We also predicted that individuals with a stronger prevention focus would tend to copy a managing behavior regardless of their earlier hedonic experience with this behavior as its recipient. These predictions were first supported in 2 experimental studies, where a stronger prevention focus was measured as a chronic disposition (Study 1) and experimentally induced as a temporary state (Study 2). Further, we tested the mechanism underlying the relation between stronger prevention and stronger copying and found that concerns about the normativeness, but not the effectiveness, of a managing behavior motivated copying for individuals with a strong prevention focus (Studies 3 and 4). We generalized these experimental results to the field by surveying a sample of superior-subordinate dyads in real world organizations (Study 5). Across all studies, we found that individuals with a stronger prevention focus tend to copy more a role model's managing behavior—independent of their hedonic satisfaction with the behavior as its recipient and their perception of its effectiveness. (PsycINFO Database Record (c) 2011 APA, all rights reserved)